Kisselev S, Kozhevnikov V
Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk, Russia
Russ. J. Immunol. 2001, July 15, 6 (2), 157-166.
In this paper we present the results of the distribution of a cell surface protein with molecular weight 104-110 kDa (p104-110) detected by monoclonal antibody made in our laboratory. The protein is expressed by a number of cell lines belonging to B lymphoid cells, but not to the other blood cell lineages. In healthy donors the expression of p104-110 is restricted to a minor population of human peripheral blood mononuclear cells shared by both CD20+ and CD20- subpopulation. No expression of p104-110 has been found on resting and activated T cells as well as on monocytes and polymorphonuclear cells. FACS analysis of mononuclear cells derived from normal human bone marrow has revealed the presence of p104-110 on committed CD34-; CD38+ mononuclear cell subset that composes only the minor part of mature CD20+ B cells being mostly CD20 negative. Furthermore, CD19+CD5+CD20- malignant cells derived from the patients with chronic lymphoid leukemia express p104-110 at high level. Similarly, it has been shown that some patients with malignant non-Hodgkin’s lymphomas can have the increased contents of CD20+ cells bearing that surface antigen. Possible associations of p104-110 expression on human B lymphoid cells with their differentiation and activation are discussed.