Yarovinsky TO, Gorlina NK, Cheredeev AN, Kozlov IG, Zorin NA*, Zorina RM*
Russian State Medical University, Moscow, Russia; *Institute for Postgraduate Training of Physician, Novokuznetsk, Russia
Russ. J. Immunol. 2001, April 15, 6 (1), 1-8.
The aim of the present study was to evaluate the influence of apha2-macroglobulin (a2M) on lymphocyte adhesion to fibroblasts. Peripheral blood lymphocytes from healthy donors and two fibroblast lines (human diploid embryo fibroblasts M-19 and mouse transformed fibroblasts L929) were used in the experiments. a2M treatment of fibroblast monolayer appeared to result in the enhancement of lymphocyte adhesion to fibroblasts. The number of attached lymphocytes was increased by 2-2.5 times. It should be noted that the effect of a2M didn’t depend on the conformational molecule changes, since either native or methylamine or plasmin transformed a2M approximately at the same fashion increased the lymphocyte adhesion to both allogeneic and xenogeneic fibroblasts. B lymphocytes were predominant cells that were attached to fibroblast monolayer without a2M treatment. However the percentage of adherent T lymphocytes was increased substantially after the fibroblast monolayer treatment by a2M. Subpopulation analysis has shown that fibroblast pretreatment by a2M didn’t result in a selective adhesion of CD4+ or CD8+ T lymphocytes, but increased the adhesiveness for both T lymphocyte subpopulations. The data obtained demonstrate that besides its participation in the processes of fibroblast adhesion a2M is capable to modify the contact interaction of these cells with lymphocytes that may have an influence on the functional consequences of this process.