Kulakov AV, Klimova SV, Yarilin DA, Rubtsova MV*, Pinegin BV, Khaitov RM
Institute of Immunology, Ministry of Health of Russian Federation, Moscow, Russia: *Lomonosov Moscow State University, Moscow, Russia
Russ. J. Immunol. 1997, December 15, 2 (3-4), 177-182.
We studied affinity of natural antibodies of human serum against glucosaminylmuramyl dipeptide (GMDP), the epitope peptidoglycan bacterial cell wall component which carries adjuvant activity. Antibodies against GMDP were isolated from the blood sera of healthy donors using thermal extraction of antibodies from specific ligand on plastic. Determination of the dissociation constant (Kd) showed equal Kd in the serum and affinity-purified anti-GMDP-antibodies, i.e. extraction by this method led to the isolation of all subpopulations of antibodies in spite of their affinity. Kd of serum, affinity-purified and monoclonal anti-GMDP-antibodies proved of low value - 10(-6) M, and according to this index anti-GMDP-antibodies may be classified between anti-protein and anti-carbohydrate antibodies.