Determination of Cross-Reactive (Common) Epitopes of Animal and Human Immunoglobulins Using Monoclonal Antibodies

Verkhovsky OA, Fedorov YuN, Sologub VK, Feoktistova TA, Ezdakova IYu

All Russia Institute of Experimental Veterinary Medicine, Moscow, Russia

Russ. J. Immunol. 1997, April 15, 2 (1), 23-28.

Monoclonal antibodies to cross-reactive epitopes of IgG and IgM of some animal species and man were produced. Specificity and cross-reactivity of the monoclonal antibodies were determined by ELISA, immunoblotting, immunodiffusion and immunoelectrophoresis. The presence of immunoglobulin epitopes common to some animal species and man that were localized on both native Ig molecules (conformational) and polypeptide chains (linear) was demonstrated. The use of cross-reactive monoclonal antibodies for quantitation and qualitation of animal and human Ig in biological fluids by different immunoassays has been showed.